Before and after we collected our rat induced blood. Our blood incubation for 3 hours, after which we took the serum that appears on the surface of blood clots. To get a good serum, the serum we centrifuge for 15 minutes at a speed of 3000 rpm. Serum obtained ready to do the next test.
On the day of induction of thyroglobulin in mice, before we took the blood, weigh and measure the body temperature. In some literature mice with autoimmune thyroiditis will experience a decrease in Graves’ Disease and Hashimoto increase in Thyroidtis. While the rise in body temperature occurs when there is a reaction caused by the induction of immunity.
Ready isolated thyroid. Thyroid then weighed and then crushed with a mortar and a few cold solution of cell membrane destruction. Then take part after the centrifuged supernatant, then added absolute ethanol in order to precipitate the protein. After waiting overnight did we obtain the protein thyroglobulin.
The first procedure we had to do to make animal models of autoimmune thyroiditis are looking thyroid from goats. We get it from the slaughter iwan in Karangploso. Thyroid we obtained then we enter the PBS solution Asida then stored in the freezer. Having considered then we are ready to isolate the protein from the thyroid to obtain thyroglobulin.